This document presents a study on iris segmentation analysis using the integro-differential operator and Hough transform techniques in biometric systems. The study experiments with two iris segmentation techniques: the integro-differential operator and Hough transform. The Hough transform technique segmented iris images more successfully than the integro-differential operator, achieving a segmentation accuracy of 80.88% compared to 22.06% for the integro-differential operator. The Hough transform also had lower false rejection and recognition error rates. However, it has higher computational complexity than the integro-differential operator. The document concludes that the Hough transform technique resulted in better overall performance than the integro-differential operator for iris segmentation