We have multiple applications hosted under same site.
All these application point to same Path, App pools and Users.
These applications are accessed with different URLs.
Like, there are three applications A, B and C, out of which C is not accessible.
Site and Apps hosted on IIS:

All the sites were working fine, but all of a sudden one of the site is done and displaying 403 error.
I have tried app pool recycle and providing full control permission to the application folder. But issue still exists.
I have compared basic settings of the sites and looks good.