Hi there,

While doing an all-to-all ANI comparison on a set of genomes, I noticed that the regular output displays different values when the genomes are switched:

1509405_PRJNA252589.fasta.gz 246200_PRJNA281.fasta.gz 76.3461 98 1679 246200_PRJNA281.fasta.gz 1509405_PRJNA252589.fasta.gz 76.9103 84 1369 

When using the --matrix option there is only a single value for this pair, which is 76.628181 (looks like the mean).

I thus have two questions:

• Why do the ANI values change when the genome are switched?
• Is there a particular reason to use the mean of the two values in the --matrix output?

Cheers,
Nils