Description:

Heterobifunctional PEG derivative that can be used to modify proteins, peptides and other materials via amino or other acid reactive chemical groups. PEGylation can increase solubility and stability and reduce immunogenicity of peptides and proteins. It can also suppress the non-specific binding of charged molecules to the modified surfaces.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, dessiccated Protect from light. Avoid frequent thaw and freeze.

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

Description:

N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, desiccate. NHS PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen.

Reaction Procedures:

Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

• Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
• PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
• Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 ⁰C. Conjugates can be purified either by size exclusion chromatography or dialysis.

ITO (Inidum Tin Oxide) Coated Coverslips

ITO (Indium Tin Oxide) coated coverslips provided from Nanocs were made by coating ITO to one side of glass coveslips. These ITO coated glass coverslips come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness around 0.15~0.17mm. ITO coverslips has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO coated glass coverslips have excellent surface roughness around +-5 nm. Thickness of our ITO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated coverslips is over 85% in the visible range.

Nanocs ITO Coated Coverslip Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Coverslip thickness: 0.15~0.17 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO coverslip size: 18 mm x 18 mm, 22 mm x 22 mm, 22 mm x 40 mm

ITO (Inidum Tin Oxide) Coated Coverslips

ITO (Indium Tin Oxide) coated coverslips provided from Nanocs were made by coating ITO to one side of glass coveslips. These ITO coated glass coverslips come with different surface resistivity from 5 ohm/sq to 100 ohm/sq, with thickness around 0.15~0.17mm. ITO coverslips has been used in LCD/OLED devices and many other devices requiring high electrical conductivity and good optical transmission. Our ITO coated glass coverslips have excellent surface roughness around +-5 nm. Thickness of our ITO coating ranges from 50 nm to 700 nm depends on their surface resistivity. Optical transmission of these ITO coated coverslips is over 85% in the visible range.

Nanocs ITO Coated Coverslip Specifications:

• Surface resistivity: 5~100 ohm/sq, see individual product label;
• Coverslip thickness: 0.15~0.17 mm;
• ITO thickness: 30~600 nm depends on ITO surface resistivity:
• Optical transmission: 83% to 90%;
• ITO coverslip size: 18 mm x 18 mm, 22 mm x 22 mm, 22 mm x 40 mm

Description:

Alexa Fluor 488 labeled Insulin is one of Nanocs fluorescent insulin conjugates. Nanocs provides a variety of chemically functionalized and bioconjugated Insulin with different functionality, including biotin Insulin, fluorescein labeled insulin, rhodamine, Cy3, Cy5, Cy5.5 and many other fluorophore labeled insulin conjugates. These functional Insulin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

• Insulin type: Recommbinant human insulin.
• Labeling ratio: 1~3 Alexa fluor 488 dyes on each Insulin molecule.
• Ex/Em wavelength: 495 nm/515 nm.
• Form: Lyophlized powder.

Storage Conditions:

• Store at 4 ⁰C or lower. Protect from light.

Description:

Maleimide PEG NHS is a heterobifunctional PEG derivative that can react with both thiol and amine groups. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. The maleimide group reacts with available –SH groups from naturally occurring or mutagenically derived cysteine residues. Maleimide groups react with sulfhydryl groups at pH 6.5~7.5 at room temperature to form stable thioether bonds.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, dessiccated. Avoid frequent thaw and freeze.

Description:

Maleimide PEG NHS is a heterobifunctional PEG derivative that can react with both thiol and amine groups. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. The maleimide group reacts with available –SH groups from naturally occurring or mutagenically derived cysteine residues. Maleimide groups react with sulfhydryl groups at pH 6.5~7.5 at room temperature to form stable thioether bonds.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C, dessiccated. Avoid frequent thaw and freeze.

Description:

Nitrophenyl functional PEG, NPC PEG is an amino (-NH₂) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with available amino groups either on their lysine residue or N-terminus. NPC PEGs react readily with amine groups at a pH of from 6.5 to 9.5.

Physical Properties:

• Off-white/white solid or viscous liquid depends on molecule weight;
• Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

• Store at -20 ⁰C. Dessicated.