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README.md

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# Public Notebooks
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# B2btools: Predict the biophysical behavior of your protein(s) - Public Notebooks
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Welcome to the repository of Jupyter Notebooks created by the Bio2Byte group!
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- [Feedaback](https://www.bio2byte.be/b2btools/feedback)
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- [Bio2Byte tools package](https://pypi.org/project/b2bTools/)
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## 📚 List of available notebooks
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## About the b2btools
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Explore the prediction-based 'biophysical variation' of proteins along its sequence. The predictions reflect 'emerging' properties, so what the sequence is capable of, not necessarily what it will do in a particular context, for example when it adopts a specific fold.
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The predicted biophysical features are the following:
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- Backbone and sidechain dynamics (DynaMine)
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- Conformational propensities (sheet, helix, coil, polyproline II) (DynaMine)
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- Early folding propensities (EFoldMine)
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- Disorder propensities (DisoMine)
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- Beta aggregation propensity (AgMata)
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- Phase seperation propensity (PPser)
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### Single Sequence analysis
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Explore prediction-based 'biophysical variation' of proteins from up to 10 sequences at the same time.
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## 📚 List of available notebooks
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#### 📦 Using PyPI (Python Package Index)
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### Single Sequence analysis
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##### 📓 Title: `Bio2ByteTools_v3_singleseq_pypi.ipynb` (latest version)
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Example of usage of our Bio2Byte tools package for single sequence file inputs (in FASTA format) using v3.0.5
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Example of usage of our Bio2Byte tools package for single sequence(s) using v3.0.5.
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3.0.5_SingleSequence_demo.ipynb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3.0.5_SingleSequence_demo.ipynb)
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#### Title: `Bio2ByteTools_v3_singleseq_pypi_with_files.ipynb` (latest version supporting FASTA files as input)
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3_singleseq_pypi_with_files.ipynb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3_singleseq_pypi_with_files.ipynb)
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##### 📓 Title: `Bio2ByteTools_v3_singleseq_pypi.ipynb` (version including PSP predictions)
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Example of usage of our Bio2Byte tools package for single sequence file inputs (in FASTA format) using v3.0.5
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3_singleseq_pypi.ipynbb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3_singleseq_pypi.ipynb)
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##### 📓 Title: `AlphaFold_+_B2B_Tools.ipynb`
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Run a set of biophysical feature predictions on top of an Alphafold thee-dimensional predicted structure.
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Run the biophysical feature predictions on top of an Alphafold thee-dimensional predicted structure.
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/AlphaFold_%2B_B2B_Tools.ipynb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/AlphaFold_%2B_B2B_Tools.ipynb)
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##### 📓 Title: `Bio2Byte_AlphaFold_Analysis.ipynb`
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From the output files generated from Alphafold, this notebook runs a set of biophysical feature prediction
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Compare the predicted biophysical features obtained with the b2btools and the pLDTT computed by Alphafold.
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/Bio2Byte_AlphaFold_Analysis.ipynb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/Bio2Byte_AlphaFold_Analysis.ipynb)
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#### 📦 Using Conda package manager
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##### 📓 Title: `Bio2ByteTools_v3.0.5_SingleSequence_demo_bioconda.ipynb` (latest version)
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Example of usage of our Bio2Byte tools package for single sequence file inputs (in FASTA format) using v3.0.5 from Bioconda channel
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- [📖 Read Jupyter Notebook](https://github.com/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3.0.5_SingleSequence_demo_bioconda.ipynb)
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- [⏯️ Open Jupyter Notebook on Google Colab](https://colab.research.google.com/github/Bio2Byte/public_notebooks/blob/main/Bio2ByteTools_v3.0.5_SingleSequence_demo_bioconda.ipynb)
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### Multiple Sequence Alignment analysis
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