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"{\"DOCNO\":\"1\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Sinorhizobium meliloti\"}},\"dataItem\":{\"description\":\"We characterized transcriptomes of a strain overexpressing syrA. Our work shows that the syrA transcriptome shares similar gene expression changes to the syrM and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression. Gene expression profiling of Sinorhizobium meliloti overexpressing syrA was performed using custom Affymetrix GeneChips\",\"title\":\"The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520401\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)\"}",
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"{\"DOCNO\":\"2\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Homo sapiens\"}},\"dataItem\":{\"description\":\"A study to define the binding loci of RelA-containing NF-kappaB dimers in a human myometrial smooth muscle cell line after exposure to TNF. Monolayers of PHM1-31 cells were exposed to TNF (10ng/ml) for 1 hour or left unstimulated. The Chromatin immunoprecipitation (ChIP) assay was performed to recover RelA-bound chromatin or non-specifically bound chromatin with IgG. That chromatin was prepared and used to probe Affymetrix GeneChIP 1.0R Human Promoter arrays. Three biological replicates of each experiment were conducted. Datasets were subsequently analysed in Partek Genomics Suite V6.6 where baseline was normalised by subtraction of IgG values from conrresponding RelA-immunoprecipitated samples. Control samples immunoprecipitated with RelA were then compared with TNF-stimulated samples immunoprecipitated with RelA.\",\"title\":\"RelA Nuclear factor-kappaB (NF-kB) Subunit binding Loci in Promoter Regions of PHM1-31 Myometrial Smooth Muscle Cells (Promoter)\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-05\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520482\",\"experimentType\":\"ChIP-chip by tiling array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"RelA Nuclear factor-kappaB (NF-kB) Subunit binding Loci in Promoter Regions of PHM1-31 Myometrial Smooth Muscle Cells (Promoter)\"}",
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"{\"DOCNO\":\"3\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Rattus norvegicus\"}},\"dataItem\":{\"description\":\"This SuperSeries is composed of the SubSeries listed below. Refer to individual Series\",\"title\":\"Aging-associated inflammatory and oxidative changes in the rat urinary bladder and dorsal root ganglia - preventive effect of caloric restriction\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520420\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Aging-associated inflammatory and oxidative changes in the rat urinary bladder and dorsal root ganglia - preventive effect of caloric restriction\"}",
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"{\"DOCNO\":\"4\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Homo sapiens\"}},\"dataItem\":{\"description\":\"To reveal the effects of carnosine on Caco-2 cells, we have employed whole genome microarray to detect genes that showed significantly different expression when exposed to carnosine. Caco-2 cells were treated with 1 mM carnosine for 3 days. Caco-2 cells were treated with 1 mM carnosine for 3 days. Three independent experiments were performed.\",\"title\":\"Gene expression profile in Caco-2 cells treated with carnosine\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520441\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Gene expression profile in Caco-2 cells treated with carnosine\"}",
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"{\"DOCNO\":\"5\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Mus musculus\"}},\"dataItem\":{\"description\":\"Mutations in methyl-CpG-binding protein 2 (MeCP2), a major epigenetic regulator, are the predominant cause of Rett syndrome. We previously found that Mecp2-null microglia are deficient in phagocytic ability, and that engraftment of wild-type monocytes into the brain of Mecp2-deficient mice attenuates pathology. We have observed that Mecp2 deficiency is associated with increased levels of histone acetylation at the cis-regulatory regions of the Mecp2-regulated genes in macrophages. We hypothesized that Mecp2 recruits protein complexes containing histone deacetylases (HDACs) to repress the expression of its target genes. Our ChIP-Seq studies in bone-marrow derived macrophages revealed that Mecp2 co-localizes with Ncor2/Hdac3 protein complex at cis-regulatory regions of the target genes. These results suggest a role for Mecp2 in the recruitment and regulation of Ncor2/Hdac3 repressosome that plays a critical role in the regulation of inflammatory responses in macrophages. Examination of NCOR2 and HDAC3 genome-wide location in bone-marrow derived macrophages.\",\"title\":\"Mecp2: an unexpected regulator of macrophage gene expression and function [ChIP-Seq]\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520444\",\"experimentType\":\"ChIP-seq\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Mecp2: an unexpected regulator of macrophage gene expression and function [ChIP-Seq]\"}" };
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"{\"DOCNO\":\"1\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Sinorhizobium meliloti\"}},\"dataItem\":{\"description\":\"We characterized transcriptomes of a strain overexpressing syrA. Our work shows that the syrA transcriptome shares similar gene expression changes to the syrM and nodD3 transcriptomes and that nodD3 and syrA may be the only targets directly activated by SyrM. We propose that most of the gene expression changes observed when nodD3 is overexpressed are due to NodD3 activation of syrM expression, which in turn stimulates SyrM activation of syrA expression. The subsequent increase in SyrA abundance alters activity of the ChvI-ExoS-ExoR circuit, resulting in broad changes in gene expression. Gene expression profiling of Sinorhizobium meliloti overexpressing syrA was performed using custom Affymetrix GeneChips\",\"title\":\"The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520401\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"The Sinorhizobium meliloti SyrM regulon: effects on global gene expression are mediated by syrA and nodD3 (SyrA)\"}",
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"{\"DOCNO\":\"2\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Homo sapiens\"}},\"dataItem\":{\"description\":\"A study to define the binding loci of RelA-containing NF-kappaB dimers in a human myometrial smooth muscle cell line after exposure to TNF. Monolayers of PHM1-31 cells were exposed to TNF (10ng/ml) for 1 hour or left unstimulated. The Chromatin immunoprecipitation (ChIP) assay was performed to recover RelA-bound chromatin or non-specifically bound chromatin with IgG. That chromatin was prepared and used to probe Affymetrix GeneChIP 1.0R Human Promoter arrays. Three biological replicates of each experiment were conducted. Datasets were subsequently analysed in Partek Genomics Suite V6.6 where baseline was normalised by subtraction of IgG values from conrresponding RelA-immunoprecipitated samples. Control samples immunoprecipitated with RelA were then compared with TNF-stimulated samples immunoprecipitated with RelA.\",\"title\":\"RelA Nuclear factor-kappaB (NF-kB) Subunit binding Loci in Promoter Regions of PHM1-31 Myometrial Smooth Muscle Cells (Promoter)\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-05\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520482\",\"experimentType\":\"ChIP-chip by tiling array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"RelA Nuclear factor-kappaB (NF-kB) Subunit binding Loci in Promoter Regions of PHM1-31 Myometrial Smooth Muscle Cells (Promoter)\"}",
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"{\"DOCNO\":\"3\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Rattus norvegicus\"}},\"dataItem\":{\"description\":\"This SuperSeries is composed of the SubSeries listed below. Refer to individual Series\",\"title\":\"Aging-associated inflammatory and oxidative changes in the rat urinary bladder and dorsal root ganglia - preventive effect of caloric restriction\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520420\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Aging-associated inflammatory and oxidative changes in the rat urinary bladder and dorsal root ganglia - preventive effect of caloric restriction\"}",
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"{\"DOCNO\":\"4\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Homo sapiens\"}},\"dataItem\":{\"description\":\"To reveal the effects of carnosine on Caco-2 cells, we have employed whole genome microarray to detect genes that showed significantly different expression when exposed to carnosine. Caco-2 cells were treated with 1 mM carnosine for 3 days. Caco-2 cells were treated with 1 mM carnosine for 3 days. Three independent experiments were performed.\",\"title\":\"Gene expression profile in Caco-2 cells treated with carnosine\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520441\",\"experimentType\":\"transcription profiling by array\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Gene expression profile in Caco-2 cells treated with carnosine\"}",
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"{\"DOCNO\":\"5\",\"METADATA\":{\"dataResource\":{\"keywords\":[],\"altNames\":[],\"acronyms\":[]},\"citation\":{\"count\":\"0\"},\"organism\":{\"experiment\":{\"species\":\"Mus musculus\"}},\"dataItem\":{\"description\":\"Mutations in methyl-CpG-binding protein 2 (MeCP2), a major epigenetic regulator, are the predominant cause of Rett syndrome. We previously found that Mecp2-null microglia are deficient in phagocytic ability, and that engraftment of wild-type monocytes into the brain of Mecp2-deficient mice attenuates pathology. We have observed that Mecp2 deficiency is associated with increased levels of histone acetylation at the cis-regulatory regions of the Mecp2-regulated genes in macrophages. We hypothesized that Mecp2 recruits protein complexes containing histone deacetylases (HDACs) to repress the expression of its target genes. Our ChIP-Seq studies in bone-marrow derived macrophages revealed that Mecp2 co-localizes with Ncor2/Hdac3 protein complex at cis-regulatory regions of the target genes. These results suggest a role for Mecp2 in the recruitment and regulation of Ncor2/Hdac3 repressosome that plays a critical role in the regulation of inflammatory responses in macrophages. Examination of NCOR2 and HDAC3 genome-wide location in bone-marrow derived macrophages.\",\"title\":\"Mecp2: an unexpected regulator of macrophage gene expression and function [ChIP-Seq]\",\"releaseDate\":\"2015-03-31\",\"lastUpdateDate\":\"2015-04-04\",\"dataTypes\":[\"organism\",\"dataItem\",\"citation\"],\"ID\":\"520444\",\"experimentType\":\"ChIP-seq\"}},\"REPOSITORY\":\"arrayexpress_020916\",\"TITLE\":\"Mecp2: an unexpected regulator of macrophage gene expression and function [ChIP-Seq]\"}"
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