Products > Capillaries, Mounts & Supplies > Capillaries > Glass Number 50 Capillaries
Glass Number 50 Capillaries
Applications
- X-ray data collection
- Liquid-liquid diffusion crystallization
- Gel acupuncture crystallization
Features
- Thin walled – 10 micron
- Glass Number 50 (Glass 0500, borosilicate)
Description
Glass Number 50 (Glass 0500, borosilicate glass) capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Glass capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/- 5 mm. Glass capillaries are available in a wide range of outside diameters from 0.1 mm to 2.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.
In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.
For 0.1 mm to 2.0 mm capillaries the open end capillary tube base size is 3.0 +/- 0.15 mm OD x 0.13 +/- 0.10 mm Wall thickness.
The Diameter is measured about 30 to 40 mm from the closed end (measuring instrument: LaserMicrometer LS 7500) The tolerances are as follows.
Diameter Tolerance Minimum Diameter Maximum Diameter
0.1 mm +/-0.05 mm 0.05 mm 0.15 mm
0.2 mm +/-0.05 mm 0.15 mm 0.25 mm
0.3 mm +/-0.05 mm 0.25 mm 0.35 mm
0.4 mm +/-0.05 mm 0.35 mm 0.45 mm
0.5 mm +/-0.05 mm 0.45 mm 0.55 mm
0.6 mm +/-0.05 mm 0.55 mm 0.65 mm
0.7 mm +/-0.05 mm 0.65 mm 0.75 mm
0.8 mm +/-0.05 mm 0.75 mm 0.85 mm
0.9 mm +/-0.05 mm 0.85 mm 0.95 mm
1.0 mm -0.05 +0.25 mm 0.95 mm 1.25 mm
1.5 mm +/-0.25 mm 1.25 mm 1.75 mm
2.0 mm +/-0.25 mm 1.75 mm 2.25 mm
Borosilicate Specifications
Glass type Borosilicate glass 3.3
Density 2.23 g/cm3
Poisson’s number 0.20
Transformation temperature Tg= 525 degrees Celsius
Annealing point approximately 560 degrees Celsius
Softening point approximately 825 degrees Celsius
Working point 1260 degrees Celsius
Refractive index 1.473 at 20 degrees Celsius; 587.6 nm
Hydrolytic resistance, class 1 (DIN ISO 719)
Acid resistance, class 1 (DIN 12 116)
Alkalai resistance, class 2 (DIN ISO 695)
Chemical composition SiO2 81.0%
Na2O 3.5%
Al2O3 2.0%
K2O 0.5%
B2O3 13.0%
Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.
Click to Zoom In
CAT NO
HR6-104
NAME
DESCRIPTION
Size: 0.1 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-106
NAME
DESCRIPTION
Size: 0.2 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-108
NAME
DESCRIPTION
Size: 0.3 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-110
NAME
DESCRIPTION
Size: 0.4 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-112
NAME
DESCRIPTION
Size: 0.5 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-114
NAME
DESCRIPTION
Size: 0.6 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-116
NAME
DESCRIPTION
Size: 0.7 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-118
NAME
DESCRIPTION
Size: 0.8 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-120
NAME
DESCRIPTION
Size: 0.9 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-122
NAME
DESCRIPTION
Size: 1.0 mm – 25 pack
PRICE
$131.00
cart quote
CAT NO
HR6-124
NAME
DESCRIPTION
Size: 1.5 mm – 25 pack
PRICE
$150.00
cart quote
CAT NO
HR6-126
NAME
DESCRIPTION
Size: 2.0 mm – 25 pack
PRICE
$164.00
cart quote
Support Material(s)
Tolerances for Capillaries
Glass Number 50 Technical Data Sheet Related Item(S)
- Capillary Cutting Stone™
- Duco Cement
- Beeswax
- Wax Pen
- Four Color Mounting Clay
- Adjustable Crystal Mount
- Brass Specimen Pin
References
MIB Buffer-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Buffers > MIB Buffer
MIB Buffer
Applications
- Crystallization grade MIB buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
MIB is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M Malonic acid / Imidazole / Boric acid (MIB) is a buffer system useful across pH 4.0 – 10.0
The MIB buffer system is composed of 0.250 M Malonic acid / 0.375 M Imidazole / 0.375 M Boric acid titrated to two stock pH‘s. 1.0 M MIB pH 4.0 is titrated using hydrochloric acid and 1.0 M MIB pH 10.0 is titrated using sodium hydroxide.
Using MIB, one can use a single buffer system to screen pH 4.0 to 10.0 by varying the ratio of MIB pH 4.0 to SPG pH 10.0. Each kit includes a 100 mL bottle of 1.0 M MIB pH 4.0, 100 mL bottle of 1.0 M MIB pH 10.0, and a user guide with a dilution table.
– 1.0 M Malonic acid / Imidazole / Boric acid pH 4.0 (0.250 M Malonic acid / 0.375 M Imidazole / 0.375 M Boric acid), titrated with Hydrochloric acid to pH 4.0 at 25° Celsius.)
– 1.0 M Malonic acid / Imidazole / Boric acid pH 4.0 (0.250 M Malonic acid / 0.375 M Imidazole / 0.375 M Boric acid), titrated with Sodium hydroxide to pH 10.0 at 25° Celsius.)
Click to Zoom In
CAT NO
HR2-495
NAME
DESCRIPTION
100 mL each of 1.0 M MIB pH 4.0 & 10.0
PRICE
$182.00
cart quote
Support Material(s)
HR2-495 1.0 M MIB SDS
1.0 M MIB pH Table Certificate Of Analysis
Related Item(S)
- Individual PACT • PACT HT Reagents
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.
MMT Buffer-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Buffers > MMT Buffer
MMT Buffer
Applications
- Crystallization grade MMT buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
MMT is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M DL-Malic acid / MES hydrate / Tris (MMT) is a buffer system useful across pH 4.0 – 9.0
The MMT buffer system is composed of 0.2 M DL-Malic acid / 0.4 M MES hydrate / 0.4 M Tris titrated to two stock pH‘s. 1.0 M MMT pH 4.0 is titrated using hydrochloric acid and 1.0 M MMT pH 9.0 is titrated using sodium hydroxide.
Using MMT, one can use a single buffer system to screen pH 4.0 to 9.0 by varying the ratio of MMT pH 4.0 to MMT pH 9.0. Each kit includes a 100 mL bottle of 1.0 M MMT pH 4.0, 100 mL bottle of 1.0 M MMT pH 9.0, and a user guide with a dilution table.
– 1.0 M DL-Malic acid / MES hydrate / Tris (MMT) pH 4.0 (0.2 M DL-Malic acid / 0.4 M MES hydrate / 0.4 M Tris, titrated with Hydrochloric acid to pH 4.0 at 25° Celsius.)
– 1.0 M DL-Malic acid / MES hydrate / Tris (MMT) pH 9.0 (0.2 M DL-Malic acid / 0.4 M MES hydrate / 0.4 M Tris, titrated with Sodium hydroxide to pH 9.0 at 25° Celsius.)
Click to Zoom In
CAT NO
HR2-499
NAME
DESCRIPTION
100 mL each of 1.0 M MMT pH 4.0 & 9.0
PRICE
$231.00
cart quote
Support Material(s)
HR2-499 1.0 M MMT SDS
1.0 M MMT pH Table Certificate Of Analysis
Related Item(S)
- Individual PACT • PACT HT Reagents
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.
PCB Buffer-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Buffers > PCB Buffer
PCB Buffer
Applications
- Crystallization grade PCB buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
PCB is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane (PCB) is a buffer system useful across pH 4.0 – 9.5.
The PCB buffer system is composed of 0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane titrated to two stock pH‘s. 1.0 M PCB pH 4.0 is titrated using hydrochloric acid and 1.0 M PCB pH 9.5 is titrated using sodium hydroxide.
Using PCB, one can use a single buffer system to screen pH 4.0 to 9.5 by varying the ratio of PCB pH 4.0 to PCB pH 10.0. Each kit includes a 100 mL bottle of 1.0 M PCB pH 4.0, 100 mL bottle of 1.0 M PCB pH 9.5, and a user guide with a dilution table.
– 1.0 Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane pH 4.0 (0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane) is titrated with Hydrochloric acid to pH 4.0 at 25° Celsius.
– 1.0 Sodium propionate / Sodium cacodylate trihydrate / BIS-TRIS propane pH 9.5 (0.4 M Sodium propionate / 0.2 M Sodium cacodylate trihydrate / 0.4 M BIS-TRIS propane) titrated with Sodium hydroxide to pH 9.5 at 25° Celsius.
Click to Zoom In
CAT NO
HR2-497
NAME
DESCRIPTION
100 mL each of 1.0 M PCB pH 4.0 & 9.5
PRICE
$380.00
cart quote
Support Material(s)
HR2-497 1.0 M PCB SDS
1.0 M PCB pH Table Certificate Of Analysis
Related Item(S)
- Individual PACT • PACT HT Reagents
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.
SPG Buffer-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Buffers > SPG Buffer
SPG Buffer
Applications
- Crystallization grade SPG buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
SPG is a novel, broad range buffer system designed to decouple the pH from buffer in crystallization and other experiments.
1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine (SPG) is a buffer system useful across pH 4.0 – 10.0
The SPG buffer system is composed of 0.125 M Succinic acid, 0.438 M Sodium phosphate monobasic monohydrate, 0.437 M Glycine titrated to two stock pH‘s. 1.0 M SPG pH 4.0 is titrated using sodium hydroxide and 1.0 M SPG pH 10.0 is titrated using sodium hydroxide.
Using SPG, one can use a single buffer system to screen pH 4.0 to 10.0 by varying the ratio of SPG pH 4.0 to SPG pH 10.0. Each kit includes a 100 mL bottle of 1.0 M SPG pH 4.0, 100 mL bottle of 1.0 M SPG pH 10.0, and a user guide with a dilution table.
– 1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine pH 4.0 (0.125 M Succinic acid / 0.438 M Sodium phosphate monobasic monohydrate / 0.437 M Glycine, titrated with sodium hydroxide to pH 4.0 at 25° Celsius.)
– 1.0 M Succinic acid / Sodium phosphate monobasic monohydrate / Glycine pH 10.0 (0.125 M Succinic acid / 0.438 M Sodium phosphate monobasic monohydrate / 0.437 M Glycine, titrated with Sodium hydroxide to pH 10.0 at 25° Celsius.)
Click to Zoom In
CAT NO
HR2-493
NAME
DESCRIPTION
100 mL of each 1.0 M SPG pH 4.0 & 10.0
PRICE
$156.00
cart quote
Support Material(s)
HR2-493 1.0 M SPG SDS
1.0 M SPG pH Table Certificate Of Analysis
Related Item(S)
- Individual PACT • PACT HT Reagents
References
1. Newman J. Novel buffer systems for macromolecular crystallization. Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):610-2. doi: 10.1107/S0907444903029640. Epub 2004 Feb 25. PMID: 14993709.
2. Newman J, Egan D, Walter TS, Meged R, Berry I, Ben Jelloul M, Sussman JL, Stuart DI, Perrakis A. Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Crystallogr D Biol Crystallogr. 2005 Oct;61(Pt 10):1426-31. doi: 10.1107/S0907444905024984. Epub 2005 Sep 28. PMID: 16204897.
PEG Smears-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Polymers > PEG Smears
PEG Smears
Applications
- Crystallization grade Polyethylene glycol Smear for formulating screens or for optimization
Features
- Mixtures of different molecular weight Polyethylene glycol′s
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
The PEG Smears come in 4 different molecular weight ranges:
• 50% PEG Smear Low (MW 400 – 1,000)
• 50% PEG Smear Medium (MW 2,000 – 5,000)
• 50% PEG Smear High (MW 6,000 – 10,000)
• 50% PEG Smear Broad (MW 400 – 10,000)
50% PEG Smear Low consist of:
• 12.5% v/v Polyethylene glycol 400
• 12.5% v/v Polyethylene glycol monomethyl ether 550
• 12.5% v/v Polyethylene glycol 600
• 12.5% w/v Polyethylene glycol 1,000
50% PEG Smear Medium consist of:
• 12.5% w/v Polyethylene glycol 2,000
• 12.5% w/v Polyethylene glycol 3,350
• 12.5% w/v Polyethylene glycol 4,000
• 12.5% w/v Polyethylene glycol monomethyl ether 5,000
50% PEG Smear High consist of:
• 16.67% w/v Polyethylene glycol 6,000
• 16.67% w/v Polyethylene glycol 8,000
• 16.67% w/v Polyethylene glycol 10,000
50% PEG Smear Broad consist of:
• 4.55% v/v Polyethylene glycol 400
• 4.55% v/v Polyethylene glycol monomethyl ether 550
• 4.55% v/v Polyethylene glycol 600
• 4.55% w/v Polyethylene glycol 1,000
• 4.55% w/v Polyethylene glycol 2,000
• 4.55% w/v Polyethylene glycol 3,350
• 4.55% w/v Polyethylene glycol 4,000
• 4.55% w/v Polyethylene glycol monomethyl ether 5,000
• 4.55% w/v Polyethylene glycol 6,000
• 4.55% w/v Polyethylene glycol 8,000
• 4.55% w/v Polyethylene glycol 10,000
50% PEG Smear Low
HR2-507
Refractive Index Range: 1.40516 – 1.40516 at 20°C
Conductivity Range: 34.7 – 34.7 µS/cm at 25°C
50% PEG Smear Medium
HR2-505
Refractive Index Range: 1.40180 – 1.40180 at 20°C
Conductivity Range: 37.4 – 37.4 µS/cm at 25°C
50% PEG Smear High
HR2-521
Refractive Index Range: 1.40224 – 1.40224 at 20°C
Conductivity Range: 36.5 – 36.5 µS/cm at 25°C
Polyethylene glycol 400
Synonyms: PEG 400
H(OCH2CH2)nOH
Mr 380-420
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3675000
MDL Number MFCD00081839
Polyethylene glycol monomethyl ether 550
Synonyms: Methoxypolyethylene glycol, mono-Methyl polyethylene glycol, Methoxy poly(ethylene glycol), PEG MME 550, MPEG
CH3(CH2CH2O)nOH
Average Mn ~550
CAS Number [9004-74-4]
EC Number 215-801-2
RTECS PE8271000
MDL Number MFCD00084416
Polyethylene glycol 600
Synonyms: PEG 600
H(OCH2CH2)nOH
Mr 570-630
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
Polyethylene glycol 1,000
Synonyms: PEG 1,000
H(OCH2CH2)nOH
Mr 950-1,050
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4025000
MDL Number MFCD00081839
Polyethylene glycol 2,000
Synonyms: PEG 2,000
H(OCH2CH2)nOH
Mr 1,900-2,200
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
Polyethylene glycol 3,350
Synonyms: PEG 3,350
H(OCH2CH2)nOH
Mr 3,015-3,685
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
MDL Number MFCD00081839
Polyethylene glycol 4,000
Synonyms: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
Polyethylene glycol monomethyl ether 5,000
Synonyms: Methoxypolyethylene glycol, mono-Methyl polyethylene glycol, Methoxy poly(ethylene glycol), PEG MME 5,000, MPEG 5,000
CH3(CH2CH2O)nOH
Average Mn 5,000
CAS Number [9004-74-4]
EC Number 215-801-2
RTECS PE8271000
MDL Number MFCD00084416
Polyethylene glycol 6,000
Synonyms: PEG 6,000
H(OCH2CH2)nOH
Mr 5,000-7,000
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4105000
MDL Number MFCD00081839
Polyethylene glycol 8,000
Synonyms: PEG 8,000
H(OCH2CH2)nOH
Mr 7,000-9,000
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4105000
MDL Number MFCD00081839
Polyethylene glycol 10,000
Synonyms: PEG 10,000
H(OCH2CH2)nOH
Mr 8,500-11,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4110000
MDL Number MFCD00081839
Click to Zoom In
CAT NO
HR2-507
NAME
DESCRIPTION
100 mL
PRICE
$88.00
cart quote
CAT NO
HR2-505
NAME
DESCRIPTION
100 mL
PRICE
$88.00
cart quote
CAT NO
HR2-521
NAME
DESCRIPTION
100 mL
PRICE
$88.00
cart quote
CAT NO
HR2-511
NAME
DESCRIPTION
100 mL
PRICE
$88.00
cart quote
Support Material(s)
HR2-507 PEG Smear Low SDS
HR2-505 PEG Smear Medium SDS
HR2-521 PEG Smear High SDS
HR2-511 PEG Smear Broad SDS Certificate Of Analysis
References
1. Chaikuad, A., Knapp, S. & von Delft, F. (2015). Defined PEG smears as an alternative approach to enhance the search for crystallization conditions and crystal-quality improvement in reduced screens. Acta Cryst. D71, 1627-1639.
Converse Concentration Devices-Hampton蛋白结晶
Products > Crystallization Plates, Hardware & Accessories > Diaplate, Dialysis Buttons, & Membranes > Converse Concentration Devices
Converse Concentration Devices
Applications
• Ultrafiltration
• Concentration and / or diafiltration for purification of biological samples
• High solute concentration solutions
• Protein and DNA desalting/buffer exchange
• Nucleic Acid concentration
• Recovery of Genomic DNA
• Removal of contaminants
Features
• No centrifuge needed with specially designed Pressure Caps
• Ready to use, just add sample
• Ideal for both small and large sample volumes ( 0.5 ml. to 20 ml.)
• No cross contamination, enclosed unit
• Specially selected low absorption membranes
• Ultrafiltration reversed preventing any membrane blockage
• Particle laden samples can be used
• Converse orientation results in very rapid filtration (10 minute protocol)
• Large dead stop, no risk of drying sample
• Multi-use if kept wet
Description
SWISSCI Converse S and L Ultrafiltration Devices have been specially designed to ensure rapid and easy concentration procedures. The reverse direction of flow combined with the large membrane surface area leads to high processing speed and excellent sample recovery. Up to 150x concentration factor possible if desired. Buffer exchange is simply achieved with Converse Devices.
No centrifuge is needed as Converse Devices can be run with specially designed Pressure Caps. Pressure Cap Results have been shown to be superior against devices run through a Centrifuge; increasing the concentration speed and factor. Centrifuge can be used if desired.
The concentration is controlled by the liquid retained in the bottom of converse outer tube and cannot go to dryness. Minimally we can give a nominal volume of 50 microlitres in the L and 20 microlitres in S devices.
SWISSCI Converse S and L both come with specially selected membranes with MWCOs of 3, 10, 30 or 100kDa. The Swissci Converse S is ideal for smaller sample solutions of up to 8ml and the Converse L is suitable for larger sample solutions of up to 20ml.
| Product | MWCO | Pack Size | Swissci Catalog # | Hampton Research Catalog # |
| Converse S | 3 kDa | 24 | V51003 | HR3-258 |
| Small | 10 kDa | 24 | V51010 | HR3-259 |
| Volume | 30 kDa | 24 | V51030 | HR3-260 |
| Concentrator | 100 kDa | 24 | V51100 | HR3-261 |
| 0.5 – 8 mL | S Pressure Cap | 4 | V5-PC4 | HR3-256 |
| Converse L | 3 kDa | 24 | V41003 | HR3-262 |
| Large | 10 kDa | 24 | V41010 | HR3-263 |
| Volume | 30 kDa | 24 | V41030 | HR3-264 |
| Concentrator | 100 kDa | 24 | V41100 | HR3-265 |
| 5 – 20 mL | L Pressure Cap | 4 | V4-PC4 | HR3-257 |
Click to Zoom In
CAT NO
HR3-256
NAME
DESCRIPTION
Pack of 4
PRICE
$80.00
cart quote
CAT NO
HR3-257
NAME
DESCRIPTION
Pack of 4
PRICE
$109.00
cart quote
CAT NO
HR3-258
NAME
DESCRIPTION
Pack of 24
PRICE
$215.00
cart quote
CAT NO
HR3-259
NAME
DESCRIPTION
Pack of 24
PRICE
$215.00
cart quote
CAT NO
HR3-260
NAME
DESCRIPTION
Pack of 24
PRICE
$215.00
cart quote
CAT NO
HR3-261
NAME
DESCRIPTION
Pack of 24
PRICE
$215.00
cart quote
CAT NO
HR3-262
NAME
DESCRIPTION
Pack of 24
PRICE
$345.00
cart quote
CAT NO
HR3-263
NAME
DESCRIPTION
Pack of 24
PRICE
$345.00
cart quote
CAT NO
HR3-264
NAME
DESCRIPTION
Pack of 24
PRICE
$345.00
cart quote
CAT NO
HR3-265
NAME
DESCRIPTION
Pack of 24
PRICE
$345.00
cart quote
Support Material(s)
Converse S Instructions For Use – Pressure Cap Operation
Converse L Instructions For Use – Pressure Cap Operation References
Hanging Drop Seal-Hampton蛋白结晶
Products > Crystallization Plates, Hardware & Accessories > Sealing Films, Tapes, Mats & Covers > Hanging Drop Seal
Hanging Drop Seal
Applications
- Hanging drop vapor diffusion
- 96 round wells
Features
- ANSI/SLAS 1-2004 Standard
- Accommodates 3 separate drops of protein per well
- Fits standard 2 or 3 drop MRC plates
- Fits Greiner 655101 microplate (Hampton Research HR3-139)
- 100 micron thickness
- Choice of UVP compatible or non-UVP compatible material
Description
The Hanging Drop Seal is compatible with the 96 Well Clear Polystyrene Flat Bottom Microplate by Griener Bio One.
The revolutionary SWISSCI Hanging Drop Seal (UVOP / Non-UVP) comes ready to use with a dust-free protective coating and specialty polymer base. The Seals accommodate up to 3 separate drops of protein and fit the standard 2-drop or 3-drop MRC plates as well as Greiner 655101 microplate (Hampton Research HR3-139). The product has a 100 micron thin layer of UVP specialty polymer developed for compact drop creation and ability to shoot X-ray without any noticeable diffraction. The non-UVP seal comes in 100-micron layer.
The SWISSCI hanging drop seals can be run with individual drops in the 96 positions or 192 drops – or 288 drops. The volume of the drop is typically between 50 and 100 nanolitres. The seal covers sit directly over the microwell plate crystallization reagent wells and are closed with a long term resistant closing adhesive which is validated not to ingress into the sample, nor lead to seal corruption.
To remove an individual crystal the seal may be peeled back or simply cut out with a knife. The material is very thin although still remaining as a perfect evaporation barrier and can be sliced with a knife very easily.
The seals are produced in a class 100,000 cleanroom environment and are dust and scratch-free. Optical quality is of the highest level available. The seals are able to be used in the UV / Non-UVP range thus enabling the user to recognize salt – from protein crystals under a UV source microscope.
Click to Zoom In
CAT NO
HR4-665
NAME
DESCRIPTION
pack of 50, SWISSCI HDP-UVP-96T02
PRICE
$370.00
cart quote
CAT NO
HR5-100
NAME
DESCRIPTION
pack of 50, SWISSCI HDP-PE-96T03
PRICE
$280.00
cart quote
Support Material(s)
Hanging Drop Seal non-UVP Datasheet
Hanging Drop Seal UVP Datasheet Related Item(S)
- X-Acto Gripster Knife
- 96 Well Clear Polystyrene Flat Bottom Microplate, Greiner 655101
References
Ethylene imine polymer-Hampton蛋白结晶
Products > Optimize Reagents > Optimize – Polymers > Ethylene imine polymer
Ethylene imine polymer
Applications
- Crystallization grade Ethylene imine polymer solution for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Ethylene imine polymer
Synonyms: PEI or Poly(ethylene imine) or Poly(ethyleneimine) solution or Ethyleneimine polymer solution
H2NCH2(C22H55N11)nCH2NH2
Mr 600,000 to 1,000,000
CAS [9002-98-6]
EC Number 205-793-9
MDL Number MFCD00084427
Sterile filtered solution
Density 1.08 g/mL at 20°C
Refractive Index N20/D: 1.440 – 1.455
Structural Viscosity: 18 – 40PAS
Appearance (Color): Faint yellow to yellow in color; clear to slightly opaque viscous liquid
Ethylene imine polymer is utilized in Crystal Screen 2 crystallization screen and StockOptions Polymer
HR2-599 Measured Refractive Index Range: 1.45032 – 1.45291 at 20°C
HR2-599 Measured Conductivity Range: 237.5 – 320.0 µS/cm at 25°C
HR2-600 Measured Refractive Index Range: 1.36837 – 1.36837 at 20°C
HR2-600Measured Conductivity Range: 52.5 – 52.5 mS/cm at 25°C
Click to Zoom In
CAT NO
HR2-599
NAME
DESCRIPTION
200 mL
PRICE
$126.00
cart quote
CAT NO
HR2-600
NAME
DESCRIPTION
100 mL
PRICE
$126.00
cart quote
Support Material(s)
HR2-599 Ethylene imine polymer SDS Certificate Of Analysis
References
1. Acta Cryst. (2004). D60, 952-953, Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus A. W. Smith, A. Camara-Artigas and J. P. Allen.
2. Acta Cryst. (2010). D66, 685-697, A crystallization screen based on alternative polymeric precipitants, C. Grimm, A. Chari, K. Reuter and U. Fischer.
Adjustable Crystal Mount-Hampton蛋白结晶
Products > Capillaries, Mounts & Supplies > Magnetic Mounts & Supports > Adjustable Crystal Mount
Adjustable Crystal Mount
Applications
- Adjustable, magnetic crystal mounting with capillaries or fibers
Features
- Adjustable Z
- Magnetic mount
- Fits XYZ Goniometer Heads with Z Platform or magnetic base
- Fast, easy, and convenient crystal positioning and retrieval
Description
The Adjustable Crystal Mount is a magnetic piece (approximately 12 mm in height, 9.8 mm in diameter) designed to position a brass specimen pin and either a capillary or glass fiber onto a goniometer head for x-ray data collection.
The Adjustable Crystal Mount is engineered to fit onto the Z Platform or magnetic base within a goniometer head. The recessed bottom properly positions the crystal mount over the center of the goniometer head and prevents the mount from sliding across the goniometer head during data collection. The bottom of the crystal mount is flat and closed to prevent the brass specimen pin from protruding through the bottom of the mount. The magnetic design allows for quick, convenient, and rapid placement and retrieval of the crystal mount. Finally, the crystal mount is designed with a single allen screw which allows the Z-translation of the brass specimen pin to be adjusted (up and down) and then secured in position by tightening the screw using a goniometer head key or 0.88 mm allen key.
Click to Zoom In
CAT NO
HR8-028
NAME
DESCRIPTION
5 pack
PRICE
$85.00
cart quote
Related Item(S)
- Glass Number 50 Capillaries
- Special Glass 10 Capillaries
- Quartz Capillaries
- Brass Specimen Pin
- Magnetic Base
